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Gibco™ Human HGF Recombinant Protein

Código de producto. 12083599
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Cantidad:
100 μg
10 μg
Tamaño de la unidad:
100 microgramos
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Código de producto. 12083599

Marca: Gibco™ PHG0324

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Descripción

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS containing 0.1% human serum albumin. Apportion the reconstituted protein into working aliquots and store at = -20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store the lyophilized protein at = -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at = -20°C. Avoid repeated freeze-thaw cycles.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P14210, Q08048
Para utilizar con (aplicación) Bioactivity
Formulación PBS with no preservative
ID de gen (Entrez) 15234, 3082
Peso molecular 70 kDa
Nombre Human HGF
Método de purificación Purified
Cantidad 10 μg
Requisitos de almacenamiento -20°C
Estado normativo RUO
Concentración de endotoxinas <0.15 EU/μg
Alias de gen C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF
Nombre común HGF
Símbolo de gen Hgf
Actividad biológica ED50 ≤ 20 ng/mL; determined by the dose-dependent proliferation of 4 MBr-5 cells.
Tipo de producto Protein
Conjugado Unconjugated
Especie Human
Recombinante Recombinant
Contenido y almacenamiento -20°C
Clasificación Carrier-Free
Actividad ED50 ≤ 20 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.
Bioensayo Proliferation of the monkey epithelial cell line 4 MBr-5
Nivel de endotoxinas <1 EU/ml
Condiciones de envío Dry Ice
Sistema de expresión HEK293 cells
Familia de proteínas Growth Factors & Receptors
Forma de proteína Recombinant, Ligand
Formulario Lyophilized
Subtipo de proteína HGF (Hepatocyte Growth Factor)
Categoría de investigación Cell Cycle & Proliferation, Oncology, Stem Cell Research, Signal Transduction, Angiogenesis
Grado de pureza o calidad >95% by SDS-PAGE
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