Gibco™ Heat Stable FGF-10 Recombinant Protein

Descripción

Gibco Human Heat Stable FGF-10 Recombinant Protein is bioactive longer than native FGF-10 in standard cell culture conditions allowing researchers to possibly use less protein to maintain the same growth rates, improving the efficiency of their cell culture applications. Gibco Human Heat Stable FGF-10 Recombinant Protein has >90% sequence homology to native FGF-10 making it a suitable replacement for native FGF-10 in most cell culture applications.

Reconstitution:

• Centrifuge the vial briefly, before opening to bring the contents to the bottom.
• Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 1.0 mg/mL.
• Alternatively, reconstitute at lower concentrations in carrier-containing medium. Do not vortex.
• Apportion the reconstituted protein into working aliquots and store at -20°C.
• Make any further dilutions of the reconstituted protein in medium, or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
• For the 5 μg unit size, reconstitute directly in carrier-containing medium.
• For other unit sizes, reconstitute to 1.0 mg/mL as follows:
  • 50 μg, reconstitute in 50 μL
  • 100 μg, reconstitute in 100 μL
  • 500 μg, reconstitute in 500 μL

Storage: Store the lyophilized protein at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C (not in a frost-free freezer) for up to 12 months. Avoid repeated freeze-thaw cycles.

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.