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GE Healthcare IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

Marca:  GE Healthcare 17-0920-07

Detalles adicionales : Peso : 0.19400kg

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Código de producto. 11534955

  • 1088.78€ / 100 ml


Consúltenos : 902 239 303 
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Descripción

Descripción

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

Especificaciones

Especificaciones

<300cm/hr.
3 to 12
IMAC Sepharose HP
4° to 30°C
Purification of Polyhistidine tagged Proteins
34μm
100mL
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