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Cytokeratin, pan Antibody (AE-1/AE-3), Novus Biologicals™

Código de producto. 18463492 Tienda Bio Techne Productos
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Cantidad:
20 ug
Tamaño de la unidad:
0.10 miligramo
20 microgramos
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18463492 20 ug 20 microgramos
18779173 - 0.10 miligramo
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Código de producto. 18463492 Proveedor Novus Biologicals N.º de proveedor NBP22942920ug

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Mouse Monoclonal Antibody has been used in 28 publications

Cytokeratin, pan Monoclonal specifically detects Cytokeratin, pan in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-reported, Single Cell Western, Dual RNAscope ISH-IHC.
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Especificaciones

Antígeno pan Cytokeratin
Aplicaciones Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), CyTOF, Western Blot, In Situ Hybridization (ISH)
Clasificación Monoclonal
Clon AE-1/AE-3
Concentración 0.2 mg/ml
Conjugado Unconjugated
Dilución Western Blot 1 - 2 ug/mL, Flow Cytometry 1 - 2 ug/ million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL, Immunohistochemistry-Paraffin 0.25 - 1.0 ug/mL, Immunohistochemistry-Frozen 0.25 - 1.0 ug/mL, Flow (Intracellular), CyTOF-reported, Single Cell Western 1:10, Dual RNAscope ISH-IHC
N.º de referencia del gen Q7Z794, Q01546
Símbolos de los genes KRT1
Especie del huésped Mouse
Inmunógeno This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Método de purificación Protein A or G purified
Cantidad 20 ug
Estado normativo RUO
Disciplina de investigación Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
Primario o secundario Primary
ID de gen (Entrez) 3848
Especificidad de la prueba Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Especies diana Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Contenido y almacenamiento Store at 4C.
Formulario Purified
Isotype IgG1 κ
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