PeproTech CXCL14 Polyclonal Antibody, PeproTech®, Invitrogen™

Descripción

AA Sequence of recombinant protein: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human BRAK (CXCL14) matrix. Sandwich ELISA: To detect Human BRAK (CXCL14) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BRAK (CXCL14) (500-P237Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BRAK (CXCL14). Western Blot: To detect Human BRAK (CXCL14) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BRAK (CXCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.