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Invitrogen™ Bovine TNF-alpha Recombinant Protein

Código de producto. 11878761
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Cantidad:
25 μg
Tamaño de la unidad:
20 microgramos
Código de producto. Cantidad unitSize
11878761 25 μg 20 microgramos
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Código de producto. 11878761

Marca: Invitrogen™ RBOTNFAI

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Descripción

Recombinant Protein

For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Activity: The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL. Expression: The mature, soluble form of bovine TNFa was expressed in Pichia pastoris, KM71H and therefore does not have endotoxin. Reconstitute with 0.5 mL of distilled water.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso Q06599
Para utilizar con (aplicación) ELISA, Immunoprecipitation, Western Blot
Formulación PBS with no preservative
ID de gen (Entrez) 280943
Peso molecular 17.4 kDa
Nombre Bovine TNF-alpha
Cantidad 25 μg
Fuente Yeast
Estado normativo RUO
Alias de gen APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1 F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Nombre común TNF alpha
Símbolo de gen TNF
Actividad biológica The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL
Conjugado Unconjugated
Especie Yeast
Recombinante Recombinant
Secuencia Bovine TNF-alpha, amino acids 77-233 (Accession # Q06599)
Contenido y almacenamiento 4°C
Sistema de expresión P. pastoris
Formulario Lyophilized
Subtipo de proteína Recombinant
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