Applied Biosystems™ Protein Thermal Shift™ Starter Kit

Descripción

Applied Biosystems™ Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.
Save Time and Money with Sample Screening: This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.
Two Kits to Choose From: The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 1000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.
Simplify Analysis with Protein Thermal Shift™ Software: Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.

• Seleccione muestras para la estabilidad térmica relativa de la proteína
• Seleccione moléculas (por ejemplo, candidatos a fármacos para moléculas pequeñas, bibliotecas basadas en fragmentos y anticuerpos) para la unión al ligando de proteínas de un modo de alto rendimiento
• Seleccione muestras para detectar cambios de estabilidad después de mutaciones o modificaciones de puntos de proteínas
• Identifique sistemáticamente las condiciones de tampón adecuadas/óptimas para la medición de las interacciones de proteína-ligando, la producción de preparados de proteínas estables, la cristalización de proteínas y el almacenamiento de proteínas
• Prepare rápidamente las proteínas de control de calidad para garantizar la integridad o pureza de las proteínas