Invitrogen™ Apolipoprotein A1 Human ELISA Kit

Descripción

The Human Apolipoprotein A1 (Hu ApoA1) ELISA quantitates Hu ApoA1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoA1. Principle of the method The Human ApoA1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La Apo A1 (apolipoproteína A1) se sintetiza en el hígado y el intestino delgado y se regula por la ingesta dietética de lípidos. Es el principal componente de las lipoproteínas humanas de alta densidad (HDL) y desempeña un papel importante en la función metabólica y de transporte de las lipoproteínas del suero. Está regulado por la ingesta dietética de lípidos, y actúa como un activador para la enzima lecitina colesterol aciltransferasa que convierte el colesterol en ésteres de colesterol. Durante la lipólisis en el tejido periférico, Apo A1 también puede desempeñar un papel como aceptor de componentes superficiales de lipoproteínas de muy baja densidad y quilomicrones. El gen que codifica Apo A1 se encuentra en el cromosoma 11.