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Eurofins DiscoverX A-Raf Protein, Active
Active, N-terminal GST-tagged, recombinant, human A-Raf, for use in Kinase Assays
Marca: Eurofins DiscoverX 14-956

Descripción
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
GenBank U01337.1 The recombinant protein contains the amino acid substitution Y301D and Y302D with reference to GenBank U01337.1. These mutations provide constitutive activation of the enzyme.
Especificaciones
mg/mL of enzyme in 40 mM Tris/HCl pH 8.0, 200 mM NaCl, 0.08 mM EGTA, 0.024% Brij-35, 4 mM glutathione, 20% (v/v) glycerol, 0.8 mM benzamidine, 0.16 mM PMSF, 0.08% 2-mercaptoethanol. Frozen solution. | |
10 μg | |
Recombinant | |
Recombinant enzyme expressed in Sf21 insect cells | |
≥47% |
65 kDa | |
Human | |
N-terminal GST | |
Truncated | |
A-Raf, active |
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