Invitrogen™ Vitronectin Monoclonal Antibody (HV2)

Mouse Monoclonal Antibody

CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse). CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands. The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.