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VEGF-165 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Código de producto. 17805293
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Cantidad:
2 x 500 μg
25 μg
50 μg
500 μg
Tamaño de la unidad:
1 miligramo
25 microgramos
50 microgramos
500 microgramos
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Código de producto. Cantidad unitSize
17805293 2 x 500 μg 1 miligramo
17825513 25 μg 25 microgramos
17805533 50 μg 50 microgramos
30054450 500 μg 500 microgramos
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Código de producto. 17805293 Proveedor Invitrogen N.º de proveedor 500P275BT1MG

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Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat VEGF 165. Anti-Rat VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat VEGF (500-P275) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat VEGF. Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P275BT-1MG will be provided as 2 x 500 μg

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno VEGF-165
Aplicaciones ELISA, Western Blot
Clasificación Polyclonal
Concentración 0.1-1.0 mg/mL
Conjugado Biotin
Formulación PBS with no preservative
génica Vegfa
N.º de referencia del gen P16612
Alias de gen H-VEGF-165, MVCD1, M-VEGF-165, vascular endothelial growth factor 165b, vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, vascular permeability factor, VEGF, VEGF164, VEGF165, Vegfa, VEGF-A, VPF
Símbolos de los genes Vegfa
Especie del huésped Rabbit
Inmunógeno E.coli-derived, 38.5 kDa Recombinant Rat VEGF 165
Método de purificación Antigen affinity chromatography
Cantidad 2 x 500 μg
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 83785
Especies diana Rat
Contenido y almacenamiento -20°C
Formulario Lyophilized
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