Invitrogen™ Kit de ELISA para lisados de células TNFRSF18 (GITR) humanos

Descripción

The Human TNFRSF18-Cell lysates (GITR) ELISA quantitates Hu GITR in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu GITR. Principle of the method The Human GITR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

El CD357 (gen relacionado con la familia del receptor del factor de necrosis tumoral inducido por glucocorticoides, GITR) es un miembro de la familia del receptor del factor de necrosis tumoral (TNFR) que se expresa en niveles bajos en macrófagos, células B y células T sin estimular. Tras la activación, las células T de CD4+ y CD8+ sobrerregulan la expresión de CD357, mientras que las células T inmunoregulatorias expresan constitutivamente altos niveles de CD357.