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Invitrogen™ TNF alpha Canine ELISA Kit
Sandwich ELISA Kit
660.00€ - 4715.00€
Especificaciones
Número de acceso | P51742 |
---|---|
Sensibilidad del ensayo | 2 pg |
ensayo | ELISA sandwich de fase sólida |
Conjugado | Biotina |
Tipo de producto | Kit de ELISA |
Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
15414883
|
Invitrogen™
ECTNF |
96 pruebas |
660.00€
1 set |
Fecha estimada de envío: 14-05-2024
Inicie sesión para ver el stock |
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17844050
|
Invitrogen™
ECTNFX10 |
10 × 96 pruebas |
4715.00€
paquete de 10 |
Fecha estimada de envío: 22-05-2024
Inicie sesión para ver el stock |
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17164819
|
Invitrogen™
ECTNFX5 |
5 x 96 pruebas |
2635.00€
cada uno |
Fecha estimada de envío: 21-05-2024
Inicie sesión para ver el stock |
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Descripción
The Canine Tumor Necrosis Factor alpha(Dog TNFα) ELISA quantitates Dog TNFα in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog TNFα. Principle of the method The Canine TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.Especificaciones
P51742 | |
ELISA sandwich de fase sólida | |
Kit de ELISA | |
Plasma, Sobrenadante, Suero | |
ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas | |
403922 | |
< 12 % | |
Placa de 96 pocillos recubiertos previamente, Estándar, Concentrado de diluyente de ensayo, Anticuerpo de detección biotinilado, SAV-HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas | |
RUO | |
50 μl de plasma; 50 μl de suero; 100 μl de sobrenadante | |
TNFAcTNF | |
1 h 20 min |
2 pg | |
Biotina | |
Quinasa e inhibidores | |
Este par de anticuerpos ELISA detecta TNF-alfa canino. Otras especies aún no determinadas. | |
Lector de microplacas colorimétrico | |
TNFA,Ctnf | |
< 10 % | |
Peroxidasa de rábano picante (HRP) | |
Matriz extracelular y adhesión celular | |
De 2 °C a 8 °C | |
Canina | |
4 h 45 min |