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TCR Vβ3 Hamster anti-Mouse, Brilliant Violet 650, Clone: KJ25, BD Optibuild™

Código de producto. 15898239
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Cantidad:
50 μg
Tamaño de la unidad:
cada uno
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15898239 50 μg cada uno
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Código de producto. 15898239 Proveedor BD Biosciences N.º de proveedor 743415

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Descripción

Armenian Hamster Monoclonal Antibody

The KJ25 antibody specifically reacts with the V β 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. V β 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 ( Mls-4[a], Mls[c] ), Mtv-3 ( Mls[c] ), Mtv-6 ( Mls-3[a], Mls[c] ), Mtv-13 ( Mls-2[a], Mls[c] ), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). V β 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of V β 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno TCR Vβ3
Clasificación Monoclonal
Clon KJ25
Concentración 0.2mg/mL
Conjugado Brilliant Violet 650
Formulación Aqueous buffered solution containing ≤0.09% sodium azide.
Alias de gen TCR V beta 3, TCR Vβ3
Especie del huésped Armenian Hamster
Inmunógeno αβ TCR purified from mouse T-cell hybridoma 2B4.6
Método de purificación Affinity Purified
Cantidad 50 μg
Estado normativo RUO
Primario o secundario Primary
Especies diana Mouse
Contenido y almacenamiento Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2 κ
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