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Applied Biosystems™ Ensayos de expresión génica TaqMan™, VIC
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Código de producto. p-7182891 Tienda Applied Biosystems Productos
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Cantidad:
L (2900 reactions), made to order
M (750 reactions), made to order
S (360 reactions), made to order
Tamaño de la unidad:
cada uno
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Solicite los ensayos de expresión génica Applied Biosystems™ TaqMan™ a través de la herramienta de búsqueda de expresión génica.

Product Code. 11927021

missing translation for 'mfr': Applied Biosystems™ 4448489

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Este producto requiere una configuración adicional

Solicite los ensayos de expresión génica Applied Biosystems™ TaqMan™ a través de la herramienta de búsqueda de expresión génica.

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Features include:

  • Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
  • Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
  • Accurate—identify small fold-changes with high accuracy of quantitation
  • Extensive content—over 1.8 million predesigned assays available for over 25 different species
  • Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
  • Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well

Approximate ship time

Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay

  • 2 unlabeled PCR primers (900 nM each final 1X concentration)
  • 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix:TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

TRUSTED_SUSTAINABILITY

Specifications

Concentración 60X
Contenido y almacenamiento 1 tubo que contiene una mezcla de 20x (tamaños S y M) o 60x (tamaño L) de ensayo preformulado (1 sonda y 2 cebadores).

Almacenar a entre -15 y -25 °C.

Método de detección Sonda de cebado
Formato Tubo
GC-Rich PCR Performance Alto
Método de PCR qPCR
Velocidad de reacción Fast
Para utilizar con (equipo) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Características ecológicas Embalaje sostenible
Línea de productos TaqMan
Tipo de producto Ensayo de expresión génica
Cantidad S (360 reactions), made to order
Condiciones de envío Temperatura ambiente
Fidelidad (frente a Taq) 60 X
N.º de reacciones 360 reacciones
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

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