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Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.

242.00€

Especificaciones

Polimerasa ADN polimerasa Taq
Tipo de producto ADN polimerasa
Para utilizar con (aplicación) Standard PCR
Concentración 5 U/µl
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Productos 2
Código de producto Marca Formato Precio Cantidad y disponibilidad  
Código de producto Marca Formato Precio Cantidad y disponibilidad  
10756421
Ver documentos Detalles de la promoción
Thermo Scientific™
EP0072
With BSA
242.00€
500 unidades
Por favor, para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo.
10829230
missing translation for 'viewDocuments' Promotion Details
Thermo Scientific™
EP0282
Without BSA
242.00€
500 unidades
Por favor, para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo.
Beschreibung

Beschreibung

Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail.

Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific priming

Features

  • Thermostable with half-life more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • DNA labeling
  • High throughput PCR

Notes

  • The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
  • The 10X Taq Buffer without Detergent is recommended for microarray experiments.
Spezifikation

Spezifikation

ADN polimerasa Taq
ADN polimerasa
Standard PCR
5 U/µl
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

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Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)

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