Invitrogen™ SAP97 Polyclonal Antibody

Descripción

PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed. PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain extract. Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining. The PA1-741 immunizing peptide corresponds to amino acids 115-133 from rat SAP97. This sequence is 84% and 74% conserved in mouse and human SAP97, respectively. PA1-741 can be used with blocking peptide PEP-055.

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.