Invitrogen™ Kit de ELISA para proteínas de unión al retinol

El ELISA competitivo de proteína de unión al retinol cuantifica la proteína de unión al retinol (RBP) en suero, plasma, orina, saliva, extractos fecales secos o medio de cultivo celular.

The Retinol Binding Protein Competitive ELISA quantitates retinol binding protein (RBP) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP independent of species. An RBP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-sheep IgG antibodies. Anti-sheep RBP antibodies and RBP-peroxidase conjugate is added to the wells. The RBP-peroxidase conjugate and any RBP in the sample will compete to bind to the anti-sheep RBP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of RBP present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.