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Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) Whole cell lysate, Non-denatured; Abnova
Marca: Abnova L024V2.200ug
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Descripción
Western Blotting, Immunoprecipitation
Especificaciones
| Immunoprecipitation, Western Blot | |
| Whole cell lysate (non-denatured) | |
| Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). | |
| 200 μg | |
| Mouse |
| Macrophage | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| Almacenar a -80°C. Dividir para evitar repetir la congelación y descongelación. |
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Título del producto
Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) Whole cell lysate, Non-denatured; Abnova > 50 μg
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