Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase

Descripción

Invitrogen™ Platinum™ Taq DNA Polymerase is a convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer. The 10X Green PCR Buffer contains a density reagent and two tracking dyes and offers the convenience of direct gel loading of PCR products.

Features of Platinum Taq Green Hot Start DNA Polymerase include:

• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Direct gel loading with green PCR buffer
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, the Taq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).

Using Platinum Taq Green Hot Start DNA Polymerase
Platinum Taq Green Hot Start DNA Polymerase is provided with the optional KB Extender to get more versatility in PCR assays with long, GC-rich, or problematic amplicons. The green PCR buffer used with Platinum Taq DNA Polymerase allows for direct gel loading of PCR products. The dyes in the green buffer do not interfere with PCR performance and are compatible with downstream applications including DNA sequencing, ligation, and restriction digestion. Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.