Thermo Scientific™ Placas con revestimiento de níquel Pierce™

Descripción

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

• Detergents used to lyse cells do not inhibit binding to Ni2+coated plates as they do with plain polystyrene
• The detection limit is 1ng of histidine fusion protein
• Better binding for more sensitive assays compared to other commercially available nickel-activated plates