Invitrogen™ Kit de ELISA competitivo de enzimas PGFM

Includes

Placas prerecubiertas, patrón, anticuerpo detector, conjugado con HRP, tampón de ensayo, tampón de lavado, cromógeno, solución de parada, cubiertas de placa y hoja de datos técnicos específicos del lote.

ELISA competitivo

The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.

Principle of the method

The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.