DiscoverX PathHunter™ eXpress TRHR Total GPCR Internalization Assay

The PathHunter™ TRHR Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. TRH) based internalization of TRHR via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress EA-tagged TRHR and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the TRHR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.