DiscoverX PathHunter™ CHO-K1 C5AR1 Bioassay Kit

The PathHunter™ C5AR1 Bioassay kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology (www.discoverx.com/technologies-platforms/enzyme-fragment-complementation-technology). The included cell line overexpresses PK-tagged C5AR1 and EA-tagged β-Arrestin 2. Activation of C5AR1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.