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Invitrogen™ PARP1 (cleaved Asp214, Asp215) Polyclonal Antibody
Rabbit Polyclonal Antibody
Marca: Invitrogen™ 44698G
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Descripción
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. The antibody has been used in western blotting and previous lots have been used in immunostaining applications. Positive controls used in western blotting were Jurkat or HeLa cells treated with staurosporine or etoposide (25 μM for 3 hours).
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
Especificaciones
| PARP1 (cleaved Asp214, Asp215) | |
| Polyclonal | |
| Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3 | |
| P09874, P11103, P18493, P27008 | |
| PARP1 | |
| The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP. | |
| 100 μL | |
| Primary | |
| Human, Mouse, Rat, Bovine | |
| Antibody | |
| IgG |
| Immunohistochemistry (Paraffin), Western Blot | |
| Unconjugated | |
| PARP1 | |
| 5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; Parp1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 11545, 142, 25591, 286764 | |
| -20°C | |
| Liquid |
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