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OPTIZYME™ DNA Polymerase I, Fisher BioReagents™
Marca: Fisher Bioreagents BP8109-1
Detalles adicionales : Peso : 0.02000kg
Descripción
OPTIZYME™ DNA Pol I is a template-dependent DNA Polymerase that catalyzes 5'->3' DNA synthesis, and exhibits 3'->5'-exonuclease, 5'->3' exonuclease and RNase H activity.
Applications and Features:
- OPTIZYME DNA Polymerase I labels DNA by nick translation in conjunction with DNase I.
- Second-strand cDNA synthesis in conjunction with RNase H.
- Incorporation of modified nucleotides (e.g., biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides).
- Active in buffers for OPTIZYME restriction enzymes and reverse transcriptases.
Supplied with:
10X OPTIZYME DNA Pol I Buffer: 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 10mM DTT
Source: E. Coli cells with a cloned polA gene for E.Coli
Quality Control:
Endodeoxyribonuclease-free
Especificaciones
| 10 U/μL | |
| 7.5 | |
| OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM | |
| E. coli cells with a cloned polA gene | |
| DNA Polymerase I |
| Colorless | |
| DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases | |
| -20°C | |
| 500 U | |
| Liquid |