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  6. Invitrogen™ OCT3/4 Recombinant Rat Monoclonal Antibody (EM92), Alexa Fluor™ Plus 488

Invitrogen™ OCT3/4 Recombinant Rat Monoclonal Antibody (EM92), Alexa Fluor™ Plus 488

Código de producto. p-200109865
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Cantidad:
20 μL
50 μL
Código de producto. Cantidad
30484983 50 μL
30484929 20 μL
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Código de producto. 30484983

Marca: Invitrogen™ 740044TP488

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Descripción

Rat Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This EM92 antibody has been tested by western blot, Immunocytochemistry and flow cytometry applications analysis of NTERA-2 cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser Filtration: 0.2 μm post-manufacturing filtered.

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno OCT3/4
Aplicaciones Flow Cytometry, Western Blot, Immunocytochemistry
Clasificación Recombinant Monoclonal
Clon EM92
Concentración 1.0 mg/mL
Conjugado Alexa Fluor Plus 488
Formulación Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
génica POU5F1
N.º de referencia del gen Q01860
Alias de gen 2-Oct; 3-Oct; 4-Oct; cb197; chunp6868; DADB-104B20.2; etID49452.21; gp9; gp-9; HGNC:9221; homeobox protein; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; MGC22487; NF-A3; Oct2; Oct-2; Oct2a; Oct2b; OCT3; Oct-3; Oct3/4; Oct-3/4; OCT4; Oct-4; Octamer-binding protein 2; octamer-binding protein 3; Octamer-binding protein 4; octamer-binding transcription factor 2; Octamer-binding transcription factor 3; OTF2; OTF-2; OTF3; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; OTTHUMP00000221150; OTTHUMP00000221151; POU class 2 homeobox 2; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain gene 2; POU domain protein 2; POU domain transcription factor OCT4; POU domain, class 2, transcription factor 2; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 3; pou2; pou-2; POU2F2; Pou5f1; pou5f1 protein; pou5f3; POU-type homeodomain-containing DNA-binding protein; spg; spg/pou2; spiel-ohne-grenzen; spiel-ohne-grenzen/pou2; wu:fd18d06
Símbolos de los genes POU5F1
Especie del huésped Rat
Método de purificación Affinity chromatography
Cantidad 50 μL
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 5460
Especies diana Human
Contenido y almacenamiento 4°C, store in dark, DO NOT FREEZE!
Tipo de producto Antibody
Formulario Liquid
Isotype IgG2a κ
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