missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ IFN gamma Human ELISA Kit

Each ready-to-use kit ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

668.00€ - 2145.00€

Especificaciones

Número de acceso P01579
Intervalo del ensayo 15,6-1000 pg/ml
Sensibilidad del ensayo <4 pg/mL
Conjugado Biotina
Tipo de producto Kit de ELISA
Ver más especificaciones

Includes: Human IFN-γ Antibody Coated 96-Well Plate
Human IFN-γ Standard
Standard Diluent Buffer
Human IFN-γ Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
Detailed protocol with validation tests
Plate precoated with capture antibody

Productos 3
Código de producto Marca Cantidad Precio Cantidad y disponibilidad  
Código de producto Marca Cantidad Precio Cantidad y disponibilidad  
10786513
Ver documentos Detalles de la promoción
Invitrogen™
KHC4021
96 pruebas
668.00€
96 tests
Ver productos alternativos
Ahorre hasta 
»
En stock
para ver el stock
Añadir a la cesta
 
10150863
Ver documentos Detalles de la promoción
Invitrogen™
KHC4022
2 x 96 pruebas
998.00€
192 tests
Ver productos alternativos
Ahorre hasta 
»
En stock
para ver el stock
Añadir a la cesta
 
10140863
Ver documentos Detalles de la promoción
Invitrogen™
KHC4021C
5 x 96 pruebas
2145.00€
paquete de 5
Ver productos alternativos
Ahorre hasta 
»
Fecha estimada de envío: 16-04-2024
para ver el stock
Añadir a la cesta
 
Descripción

Descripción

The Human Interferon-gamma (Hu IFN-γ) ELISA quantitates Hu IFN-γ in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFN-γ. Principle of the method The Human IFN-γ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Detects and quantifies the level of human IFN-γ in serum and supernatant using 96-well plates and a microplate reader. Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run. Easy-to-run sandwich ELISA has a total assay incubation time is only 2.75 hours. A monoclonal capture antibody specific for Human IFN-γ machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human IFN-γ content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Human IFN-γ antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human IFN-γ present in the original specimen and the optical density can be read on a standard microplate reader.

See protocol insert for more information on validation.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Información de órdenes

Condición de envío:Temperatura ambiente

Especificaciones

Especificaciones

P01579
<4 pg/mL
Kit de ELISA
Humano
Lector de microplacas colorimétrico
IFG, IFI
6,0%
Placa de 96 pocillos recubiertos previamente, Estándar, Tampón de dilución estándar, Anticuerpo de detección biotinilado, Estreptavidina-HRP, Diluyente HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas
IFN-γ
Suero, 50 μl; Sobrenadante, 50 μl
Humano
2 h 45 min
15,6-1000 pg/ml
Biotina
Sobrenadante, Suero
Detección colorimétrica
3458
2,75 h
5,5%
Peroxidasa de rábano picante (HRP)
RUO
De 2 °C a 8 °C
1 h 20 min
Videos
SDS
Documentos

Documentos

Product Certifications

Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.