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Invitrogen™ nNOS Polyclonal Antibody
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Código de producto. 11571442
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Cantidad:
100 μg
Tamaño de la unidad:
100 microgramos
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11571442 100 μg 100 microgramos
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Código de producto. 11571442 Proveedor Invitrogen™ N.º de proveedor PA1033

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Descripción

Rabbit Polyclonal Antibody

PA1-033 detects nNOS from human, rat and mouse samples. PA1-033 has been successfully used in Western blot, ICC/IF and immunohistochemisty (paraffin) procedures. By Western blot, this antibody detects an ~160 kDa protein. Detects a few non-specific bands in Western blot analysis of rat and mouse brain lysates. This antibody is not recommended for MCF-7, PC-12, SK-N-C or U87-MG cells in Western blot applications. The PA1-033 immunogen is a synthetic peptide to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, mouse, frog, and rabbit nNos proteins. This peptide (Cat. # PEP-190) is available for use in neutralization and control experiments.

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno nNOS
Aplicaciones Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Clasificación Polyclonal
Concentración 1 mg/mL
Conjugado Unconjugated
Formulación PBS with 1mg/mL BSA and 0.05% sodium azide
génica NOS1
N.º de referencia del gen P29475, P29476, Q9Z0J4
Alias de gen 2310005C01Rik; bNOS; bNOS1; Brain NOS; Constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; neuronal nitric oxide synthase NOS1; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Símbolos de los genes NOS1
Especie del huésped Rabbit
Inmunógeno Synthetic peptide corresponding to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS.
Método de purificación Antigen affinity chromatography
Cantidad 100 μg
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 18125, 24598, 4842
Especies diana Human, Mouse, Rat
Contenido y almacenamiento -20°C, Avoid Freeze/Thaw Cycles
Tipo de producto Antibody
Formulario Liquid
Isotype IgG
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