Invitrogen™ N-cadherin Monoclonal Antibody (NCD-2)

Rat Monoclonal Antibody

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of Wistar rat after immunization with nervous tissue of chick embryos. The monoclonal antibody was harvested from ascitic fluid of SCID mouse. Specificity: This antibody specifically reacts with chicken N-cadherin. This antibody inhibits N-cadherin dependent cell-cell contact. Cross reactivity: This antibody reacts with Xenopus N-cadherin. This antibody does not react with human or mouse N-cadherin. Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C. Ca2+ should be contained in the buffers t...