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  5. Invitrogen™ Mouse IL-5 ELISA Kit

Invitrogen™ Mouse IL-5 ELISA Kit

Código de producto. p-200115243
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Cantidad:
5 x 96 pruebas
Tamaño de la unidad:
cada uno
paquete de 10
Código de producto. Cantidad unitSize
18587774 5 x 96 pruebas paquete de 10
17175749 5 x 96 pruebas cada uno
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Código de producto. 18587774

Marca: Invitrogen™ EMIL5ALPHAX10

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Descripción

ELISA

The Mouse Interleukin-5 (Ms IL-5) ELISA quantitates Ms IL-5 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-5. Principle of the method The Mouse IL-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; Cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; Il5; IL-5; ILN; Interleukin; interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF
Intervalo del ensayo 2.05 to 500 pg/mL
Sensibilidad del ensayo 1 pg/mL
Conjugado HRP
Tipo de producto ELISA
Tipo de muestra Plasma, Serum, Supernatant
Para utilizar con (equipo) Colorimetric Microplate Reader
ID de gen (Entrez) 16191
Símbolo de gen Il5
CV interensayo <12%
CV intraensayo <10%
Contenido del kit Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Etiqueta o tinte HRP
Cantidad 5 x 96 pruebas
Estado normativo RUO
Volumen de muestra Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Requisitos de almacenamiento 2°C to 8°C
Objetivo IL-5
Tiempo de prueba 1 hr. 20 min.
Tiempo total del ensayo 4 hr. 45 min.
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