Invitrogen™ Molecular Probes™ Oregon Green™ 514 Phalloidin

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green 514 provides green fluorescence with brightness superior to fluorescein. Oregon Green 514 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green 514 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green 514 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green 514 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot™ nanocrystals, and Alexa Fluor conjugates including secondary antibodies.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 511/528nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• pH insensitive under physiological conditions

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cytoskeleton, Organelle Tracing