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PeproTech Midkine Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Descripción
AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMidkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P171BT-1MG will be provided as 2 x 500 μg
Especificaciones
Especificaciones
| Antígeno | Midkine |
| Aplicaciones | ELISA, Western Blot |
| Clasificación | Polyclonal |
| Concentración | 0.1-1.0 mg/mL |
| Conjugado | Biotin |
| Formulación | PBS with no preservative |
| génica | Mdk |
| N.º de referencia del gen | P21741 |
| Alias de gen | amphiregulin-associated protein; ARAP; FLJ27379; MDK; Mek; Midgestation and kidney protein; midkine; midkine (neurite growth-promoting factor 2); MK; MK1; NEGF2; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; Retanoic acid-responsive protein; retinoic acid inducible factor; retinoic acid-induced differentiation factor; retinoic acid-responsive protein |
| Símbolos de los genes | Mdk |
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