PeproTech MCP-3 Polyclonal Antibody, PeproTech®, Invitrogen™

Descripción

AA Sequence of recombinant protein: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human MCP-3 (CCL7). Anti-Human MCP-3 (CCL7)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-3 (CCL7) matrix. Sandwich ELISA: To detect Human MCP-3 (CCL7) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-3 (CCL7) (500-P37GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MCP-3 (CCL7). Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.