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  6. Invitrogen™ LysoLight™ Antibody Labeling Kits

Invitrogen™ LysoLight™ Antibody Labeling Kits

Código de producto. 30245199
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Cantidad:
3 x 100 μg
3 x 1 mg
500 μg
Tipo de producto:
Amine Reactive Dye
Antibody Labeling Kit
Color:
Deep Red
Verde
Tamaño de la unidad:
0.50 miligramo
1 miligramo
300 microgramos
cada uno
paquete de 3
8 product options available for selection
Product selection table with 8 available options. Use arrow keys to navigate and Enter or Space to select.
Código de producto. Cantidad Tipo de producto Color unitSize
30245199 3 x 100 μg Amine Reactive Dye Verde paquete de 3
18051853 3 x 100 μg Antibody Labeling Kit Deep Red cada uno
18041853 3 x 1 mg Antibody Labeling Kit Deep Red cada uno
18014120 3 x 100 μg Amine Reactive Dye Deep Red 300 microgramos
18034070 500 μg Amine Reactive Dye Deep Red 0.50 miligramo
30245367 3 x 100 μg Antibody Labeling Kit Verde cada uno
30245299 3 x 1 mg Antibody Labeling Kit Verde cada uno
30245107 500 μg Amine Reactive Dye Verde 1 miligramo
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Artikelnummer. 30245199 Leverandør Invitrogen™ Leverandørnr. L36007

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Beskrivelse

The LysoLight Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight dyes. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.

LysoLight Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight dyes have little to no observable background fluorescence, and only upon localization to the protease rich environment of the lysosome, does LysoLight become activated and brightly fluoresce bright green or deep red in color.

The labeling protocol provided in the LysoLight Antibody Labeling Kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight dye, buffers, and purification columns, to facilitate the antibody labeling process. The LysoLight reactive dye contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 μg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive form of the LysoLight dye for labeling either 100 μg or 1 mg of IgG.
TRUSTED_SUSTAINABILITY

Tekniske data

Color Verde
Método de detección Fluorescente
Para utilizar con (aplicación) Antibody and Protein Labeling
Para utilizar con (equipo) Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Tipo de producto Amine Reactive Dye
Características ecológicas Enhanced stability
Línea de productos LysoLight
Cantidad 3 x 100 μg
Solubilidad DMSO (dimetilsulfóxido)
Excitación/emisión 488/525 nm

Para uso exclusivo en investigación.

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