Invitrogen™ Kit de ELISA para LIX/CXCL5 en ratón

Descripción

The Mouse LIX (CXCL5) ELISA quantitates Ms LIX in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms LIX. Principle of the method The Mouse LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL5 es miembro de la subfamilia de quimiocinas CXC. El ADNc codifica una proteína precursora de 114 aminoácidos que, cuando se escinde, genera un péptido señal de 36 aminoácidos y una proteína madura de 78 aminoácidos que contiene una secuencia de 12 aminoácidos que se encuentra antes del motivo CXC. La CXCL5 es producida por varias células epiteliales humanas y se une a los mismos receptores que la IL8 y la NAP2.