Invitrogen™ Levonorgestrel (LNG) Competitive ELISA Kit

Includes

Placas prerecubiertas, patrón, anticuerpo detector, conjugado con HRP, tampón de ensayo, tampón de lavado, cromógeno, solución de parada, cubiertas de placa y hoja de datos técnicos específicos del lote.

ELISA

The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.

Principle of the method

The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.