Platinum™, SYBR™ GreenER™ SYBR GreenER™ qPCR SuperMix Universal

Includes

2X SYBR GreenER qPCR SuperMix and a separate tube of ROX reference dye

Incorpore la última tecnología de alto rendimiento para proporcionar los datos de expresión génica más fiables en una variedad de plataformas, tales como ABI 7500, Corbett Rotor-Gene™, etc.

SYBR™ GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR™ GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with. A novel dsDNA-binding dye exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR™ Green I, while using the same instrument filters and settings. An optimized buffer system improves sensitivity and provides excellent long-term stability. Uracil DNA glycosylase reduces carryover contamination in qPCR.

Eight cDNA targets and eight genomic DNA targets were amplified from 1ng of cDNA and 6pg of gDNA, respectively, on the ABI PRISM™ 7900HT Sequence Detection System using the SYBR™ GreenER™ qPCR SuperMix for ABI PRISM™ instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.