Invitrogen™ IGF1R (CD221) Monoclonal Antibody (1H7)

Description

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is pancreas or placenta. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Human CD221 antibody, clone 1H7 recognizes human CD221, a approximately 155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor).

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.