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Invitrogen™ Kit de ELISA para IL-6 humana
Descripción
The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target: Natural and recombinant human interleukin-6 (IL-6)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.2 to 400pg/mL
Sensitivity: < 1pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Especificaciones
Especificaciones
| Intervalo del ensayo | 10.2 to 400pg/mL |
| Sensibilidad del ensayo | <1pg/mL |
| Descripción | Human IL-6 ELISA Kit |
| Formato del kit de inmunoensayo | Colorimetric ELISA (TMB substrate) with Plates |
| Tipo de muestra | Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine |
| Especificidad | Sin reacción cruzada significativa con IL-1a humano, IL-1ß, IL-2, IL-3, IL-4, Il-5, IL-7, IL-8, IL-10, IL-12, TNFA, IFNg, IFNa, GM-CSF o IL-6 de ratón |
| Suficiente para | 2 x 96 microplate wells |
| Contenido del kit | Biotinylated Antibody Reagent, 2 x 8mL, Anti-Human IL-6 Precoated 96-well Strip Plate, 2 ea, Adhesive Plate Covers, 12 ea, 30X Wash Buffer, 2 x 50mL, HRP Dilution Buffer, 2 x 14mL, Recombinant Human IL-6 Standard, 4 vials, Standard Diluent, 2 x 25mL, Stop Solution, 2 x 13mL, TMB Substrate Solution, 2 x 13mL |
| Medición | Absorbancia a 450 nm menos absorbancia a 550 nm |
| Precisión | CV del intraensayo <10 %; CV del interensayo <10 % |
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