Invitrogen™ HTRA2 (OMI) Human ELISA Kit

Descripción

The Human HTRA2(OMI) ELISA quantitates Hu OMI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu OMI. Principle of the method The Human OMI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La familia de proteínas inhibidoras de la apoptosis (IAP) regula la muerte celular programada desencadenada por diversos estímulos. Todas las IAP tienen al menos un motivo de repetición de IAP de baculovirus (BIR) que es esencial para su actividad antiapoptótica (1-3). Recientemente, se ha aislado una serina proteasa, que se libera de la mitocondria tras la inducción de apoptosis por estímulos apoptóticos. Esta proteína llamada OmI/HtrA2 es una proteína mitocondrial que se une a IAP (4). Al igual que Smac/DIABLO, la proteína Omi madura contiene un motivo conservado que se une a IAP (AVPS) en su extremo N. La expresión desregulada de Omi en el citoplasma de las células de mamíferos induce apoptosis, lo que indica que podría participar en la vía apoptótica mitocondrial.