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Thermo Scientific™ GFP-4E-BP1
Descripción
4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (Cat. No. PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
Specifications
Specifications
| Entrada del ensayo | Actividad bioquímica fosfoespecífica del terbio |
| Contenido y almacenamiento | Sustrato de proteínas GFP-4E-BP1 en 10 mm de Tris, pH 7,5, 150 mm de NaCl, 10 % de glicerol, DTT de 2 mm. Almacenar a -80 °C. |
| Método de detección | Fluorescente |
| Formulario | Mediciones |
| Para utilizar con (aplicación) | Industria farmacéutica y biofarmacéutica, ensayos y análisis de proteínas, identificación y validación de dianas y secuencias líder |
| Cantidad | 20 nmol |
| Sustrato | 4E-BP1 |
| Substrate Properties | Sustrato basado en proteínas |
| Tipo de sustrato | Sustrato de cinasa |
| Target Enzyme | Serina/treonina cinasa |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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