PeproTech FGF10 Polyclonal Antibody, PeproTech®, Invitrogen™

Beschreibung

AA Sequence of recombinant protein: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-10 matrix. Sandwich ELISA: To detect Human FGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-10 (500-P151GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-10. Western Blot: To detect Human FGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.