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  6. Invitrogen™ F4/80 Monoclonal Antibody (Cl:A3-1)

Invitrogen™ F4/80 Monoclonal Antibody (Cl:A3-1)

Código de producto. 15245899
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100 μg
250 μg
500 μg
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100 microgramos
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Código de producto. Cantidad unitSize
15245899 500 μg 500 microgramos
15255899 100 μg 100 microgramos
15275899 250 μg 250 microgramos
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Código de producto. 15245899

Marca: Invitrogen™ MA516629

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Descripción

Rat Monoclonal Antibody

This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hrs. Citrate buffer (pH 6.0) is recommended for tissues fixed for more than 24 hrs. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno F4/80
Aplicaciones Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
Clasificación Monoclonal
Clon Cl:A3-1
Concentración 1 mg/mL
Conjugado Unconjugated
Formulación PBS with 0.09% sodium azide
génica ADGRE1
N.º de referencia del gen Q61549
Alias de gen ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
Símbolos de los genes ADGRE1
Especie del huésped Rat
Inmunógeno Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Método de purificación Protein G
Cantidad 500 μg
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 13733
Especies diana Mouse
Contenido y almacenamiento Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Tipo de producto Antibody
Formulario Liquid
Isotype IgG2b
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