Invitrogen™ EPO Receptor Human ELISA Kit

Descripción

The Human EPO Receptor (EPO R) ELISA quantitates Hu EPO R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EPO R. Principle of the method The Human EPO R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

El receptor recombinante de eritropoyetina humana (EPO R) es una proteína transmembranal de tipo I, expresada en una línea celular de mieloma de ratón, NSO. Este producto se produce a partir de una secuencia de ADNc que codifica el dominio extracelular del receptor de la EPO. El receptor de la EPO recombinante soluble de residuos aminoácidos 225 maduro que se genera después de la división de un péptido señal de residuos de aminoácidos 25 tiene una masa molecular prevista de aproximadamente 25 kDa.