Invitrogen™ Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor™ 488

Descripción

The anti-DNP-KLH antibody is supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, containing 5 mM sodium azide. The degree of labeling is indicated on the product label. This antibody was raised in rabbit against DNP conjugated to keyhole limpet hemocyanin. The IgG fraction, which contains both anti-DNP and anti-KLH IgGs, was isolated from total serum and determined to be >95% pure by SDS-polyacrylamide gel electrophoresis. It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Peak absorption and fluorescence emission wavelengths for Alexa Fluor™ 488 anti-DNPKLH conjugate is 494 nm and 520 nm, respectively Because of their high affinity for the dinitrophenyl hapten1,2 (DNP), Invitrogen's antibodies against the dinitrophenyl- keyhole limpet hemocyanin conjugate (anti-DNP-KLH) are sensitive probes for detecting DNP-labeled molecules. The fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibod...

DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).