missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Applied Biosystems™ Detection Enhancer, for real-time PCR
Formulated to improve template amplification in real-time PCR (qPCR) workflows
12.58€ - 98.75€
Especificaciones
Método de detección | Sonda de cebado |
---|---|
GC-Rich PCR Performance | High |
Método de PCR | 1-step RT-qPCR |
Velocidad de reacción | Estándar |
Para utilizar con (equipo) | Sistema 7500 |
Código de producto | Marca | N.º de reacciones | Precio | Cantidad y disponibilidad | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Código de producto | Marca | N.º de reacciones | Precio | Cantidad y disponibilidad | ||||||
30057782
|
Applied Biosystems™
A44811 |
1000 x 25 μL Reactions |
98.75€
1000 reacciones |
Fecha estimada de envoi: 15-07-2025
Inicie sesión para ver el stock |
¿Ya tiene una cuenta? Inicie sesión para ver su precio y disponibilidad. ¿Es nuevo aquí? ¡Regístrese hoy mismo! |
|||||
30057791
|
Applied Biosystems™
A44941 |
500 x 25 μL Reactions |
59.50€
500 reacciones |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||||
30054472
|
Applied Biosystems™
A44810 |
100 x 25 μL Reactions |
12.58€
100 reacciones |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||||
Descripción
- Detection Enhancer is uniquely formulated to improve template amplification in real-time PCR (qPCR) workflows. This reagent is particularly beneficial with DNA samples containing GC-rich sequences and/or persistent secondary structure. Detection Enhancer is a component of the AgPath-ID One-Step RT-qPCR Reagents kit and is offered here as a stand-alone product that can be used with any Applied Biosystem real-time PCR master mix.
- Improve qPCR amplification of templates containing high GC content and persistent secondary structure
- Compatible with any Applied Biosystem real-time PCR master mix
- Flexible kit sizes for a range of laboratory throughputs
- GC-rich DNA sequences are usually defined as those that contain >60% cytosine (C) or guanine (G). GC-rich samples can be particularly challenging to amplify because the sequences are more stable than those with lower GC content. In qPCR experiments, this results in a higher DNA melting point. In addition, GC-rich sequences can form secondary structure, which can add further amplification challenges. Adding a small volume of Detection Enhancer to your reaction mix can dramatically improve qPCR amplification in GC-rich samples.
- Detection Enhancer is specifically formulated to address qPCR amplification in particularly stable DNA sequences. It is only for use in real-time PCR workflows where amplification is affected by templates that are GC rich and/or contain persistent secondary structure. The reagents may affect sensitivity for other target types, so it is recommended to first assemble the reaction without Detection Enhancer. If no signal is observed from samples that are expected to be positive, then add Detection Enhancer to the reaction.
- The recommended volume of Detection Enhancer per 25 μL reaction is 1.67 μL. For more information on applications of Detection Enhancer, please see the AgPath-ID One-Step RT-qPCR Reagents kit user guide
Especificaciones
Sonda de cebado | |
1-step RT-qPCR | |
Sistema 7500 | |
Detection Enhancer | |
Real Time PCR (qPCR) |
High | |
Estándar | |
Applied Biosystems™ | |
Dry Ice | |
Viral Samples |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
¿Detecta una oportunidad de mejora?Comparta una corrección de contenido
Corrección del contenido de un producto
Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.
Título del producto
Applied Biosystems™ Detection Enhancer, for real-time PCR