CD42b Monoclonal Antibody (HIP1), NovaFluor™ Blue 610-70S, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb. Applications Reported: This HIP1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single color compensation controls using cells. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser