Invitrogen™ CD28 Recombinant Mouse Monoclonal Antibody (CD28.2), Invitrogen™

Mouse Recombinant Monoclonal Antibody

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency. Description: The CD28.2 recombinant mouse monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: The CD28.2 recombinant mouse monoclonal antibody has been reported for use in flow cytometric analysis. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays. Applications Tested: The CD28.2 recombinant mouse monoclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 0.025 μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.