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CD272 (BTLA) Mouse anti-Human, Brilliant Violet 605, Clone: J168-540, BD Optibuild™

Código de producto. 15818689
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Cantidad:
50 μg
Tamaño de la unidad:
cada uno
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15818689 50 μg cada uno
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Product Code. 15818689 missing translation for 'mfr' BD Biosciences missing translation for 'supplierNo' 743986

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Mouse Monoclonal Antibody

The J168-540 monoclonal antibody specifically binds to human CD272, also known as BTLA (B and T lymphocyte attenuator), an inhibitory receptor expressed in bone marrow and thymus on developing B and T cells. In the periphery, BTLA is expressed by B cells, T cells, bone marrow-derived dendritic cells, and macrophages. After T cell activation, BTLA appears to be expressed at higher levels on Th1 cell populations than in Th2 cell populations. Upon binding the herpesvirus entry mediator (HVEM/LIGHT-R/CD270), CD272 undergoes tyrosine phosphorylation and inhibits T cell proliferation in a BTLA-dependent manner. CD272 has structural similarities to two other lymphocyte inhibitory receptors, CTLA-4 and PD-1 and is a member of the CD28-like family of coreceptors. Based on these observations, CD272 is considered to be a negative regulator of lymphocyte activation and/or function.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
TRUSTED_SUSTAINABILITY

Specifications

Antígeno CD272 (BTLA)
Clasificación Monoclonal
Clon J168-540
Concentración 0.2mg/mL
Conjugado Brilliant Violet 605
Formulación Aqueous buffered solution containing ≤0.09% sodium azide.
Alias de gen BTLA1, B- and T-lymphocyte-associated protein
Especie del huésped Mouse
Inmunógeno Human BTLA Recombinant Protein
Método de purificación Affinity Purified
Cantidad 50 μg
Estado normativo RUO
Primario o secundario Primary
Especies diana Human
Contenido y almacenamiento Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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